Comparison of Two Assays for Molecular Determination of Rifampin Resistance in Clinical Samples from Patients with Buruli Ulcer Disease

Medientyp: E-Artikel
Titel: Comparison of Two Assays for Molecular Determination of Rifampin Resistance in Clinical Samples from Patients with Buruli Ulcer Disease
Beteiligte: Jansson, Moritz; Beissner, Marcus; Phillips, Richard Odame; Badziklou, Kossi; Piten, Ebekalisai; Maman, Issaka; Sarfo, Fred Stephen; Huber, Kristina Lydia; Rhomberg, Agata; Symank, Dominik; Wagner, Magdalena; Wiedemann, Franz; Nitschke, Jörg; Banla Kere, Abiba; Herbinger, Karl-Heinz; Adjei, Ohene; Löscher, Thomas; Bretzel, Gisela
Erschienen: American Society for Microbiology, 2014
Erschienen in: Journal of Clinical Microbiology
Sprache: Englisch
DOI: 10.1128/jcm.03119-13
ISSN: 1098-660X; 0095-1137
Schlagwörter: Microbiology (medical)
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Beschreibung: <jats:title>ABSTRACT</jats:title> <jats:p> This study evaluates a novel assay for detecting rifampin resistance in clinical <jats:named-content content-type="genus-species">Mycobacterium ulcerans</jats:named-content> isolates. Although highly susceptible for PCR inhibitors in 50% of the samples tested, the assay was 100% <jats:named-content content-type="genus-species">M. ulcerans</jats:named-content> specific and yielded >98% analyzable sequences with a lower limit of detection of 100 to 200 copies of the target sequence. </jats:p>
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