• Medientyp: E-Artikel
  • Titel: Real-Time PCR Assay for Rapid Detection of Epidemiologically and Clinically Significant Mycobacterium tuberculosis Beijing Genotype Isolates
  • Beteiligte: Mokrousov, Igor; Vyazovaya, Anna; Zhuravlev, Viacheslav; Otten, Tatiana; Millet, Julie; Jiao, Wei-Wei; Shen, A-Dong; Rastogi, Nalin; Vishnevsky, Boris; Narvskaya, Olga
  • Erschienen: American Society for Microbiology, 2014
  • Erschienen in: Journal of Clinical Microbiology
  • Sprache: Englisch
  • DOI: 10.1128/jcm.03193-13
  • ISSN: 0095-1137; 1098-660X
  • Schlagwörter: Microbiology (medical)
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  • Beschreibung: <jats:title>ABSTRACT</jats:title> <jats:p> <jats:named-content content-type="genus-species">Mycobacterium tuberculosis</jats:named-content> Beijing genotype strains are rapidly disseminating, frequently hypervirulent, and multidrug resistant. Here, we describe a method for their rapid detection by real-time PCR that targets the specific IS <jats:italic>6110</jats:italic> insertion in the <jats:italic>dnaA-dnaN</jats:italic> genome region. The method was evaluated with a geographically and genetically diverse collection representing areas in East Asia and the former Soviet Union in which the Beijing genotype is endemic and epidemic (i.e., major foci of its global propagation) and with clinical specimens. </jats:p>
  • Zugangsstatus: Freier Zugang