> Detailanzeige

Underwood, Sarah; Guan, Shuang; Vijayasubhash, Vinod; Baines, Simon D.; Graham, Luke; Lewis, Richard J.; Wilcox, Mark H.; Stephenson, Keith

Characterization of the Sporulation Initiation Pathway of Clostridium difficile and Its Role in Toxin Production

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Characterization of the Sporulation Initiation Pathway of Clostridium difficile and Its Role in Toxin Production
  • Beteiligte: Underwood, Sarah; Guan, Shuang; Vijayasubhash, Vinod; Baines, Simon D.; Graham, Luke; Lewis, Richard J.; Wilcox, Mark H.; Stephenson, Keith
  • Erschienen: American Society for Microbiology, 2009
  • Erschienen in: Journal of Bacteriology, 191 (2009) 23, Seite 7296-7305
  • Sprache: Englisch
  • DOI: 10.1128/jb.00882-09
  • ISSN: 0021-9193; 1098-5530
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Clostridium difficile</jats:italic> is responsible for significant mortality and morbidity in the hospitalized elderly. <jats:italic>C. difficile</jats:italic> spores are infectious and are a major factor contributing to nosocomial transmission. The Spo0A response regulator is the master regulator for sporulation initiation and can influence many other cellular processes. Using the ClosTron gene knockout system, we inactivated genes encoding Spo0A and a putative sporulation-associated sensor histidine kinase in <jats:italic>C. difficile</jats:italic> . Inactivation of <jats:italic>spo0A</jats:italic> resulted in an asporogeneous phenotype, whereas inactivation of the kinase reduced <jats:italic>C. difficle</jats:italic> sporulation capacity by 3.5-fold, suggesting that this kinase also has a role in sporulation initiation. Furthermore, inactivation of either <jats:italic>spo0A</jats:italic> or the kinase resulted in a marked defect in <jats:italic>C. difficile</jats:italic> toxin production. Therefore, Spo0A and the signaling pathway that modulates its activity appear to be involved in regulation of toxin synthesis in <jats:italic>C. difficile</jats:italic> . In addition, Spo0A was directly phosphorylated by a putative sporulation-associated kinase, supporting the hypothesis that sporulation initiation in <jats:italic>C. difficile</jats:italic> is controlled by a two-component signal transduction system rather than a multicomponent phosphorelay. The implications of these findings for <jats:italic>C. difficile</jats:italic> sporulation, virulence, and transmission are discussed. </jats:p>
  • Zugangsstatus: Freier Zugang