Beschreibung:
<jats:p>
The effects of bacteriocin JF246, produced by
<jats:italic>Serratia marcescens</jats:italic>
, on the incorporation of labeled leucine and thymidine, the synthesis of β-galactosidase, the active transport of labeled leucine and α-methyl-
<jats:sc>d</jats:sc>
-glucoside, and the cellular levels of adenosine triphosphate (ATP) in
<jats:italic>Escherichia coli</jats:italic>
were studied. This bacteriocin strongly inhibited the incorporation of leucine and thymidine into protein and deoxyribonucleic acid, respectively, as well as the active transport of leucine. The accumulation of α-methyl-
<jats:sc>d</jats:sc>
-glucoside, which is mediated by a phosphoenolpyruvate-dependent phosphotransferase system, was not markedly inhibited. The level of ATP in bacteriocin-treated cells rapidly fell to 10 to 15% of the control value. However, the kinetics of inhibition of macromolecular synthesis by various levels of bacteriocin was not related to the kinetics of ATP decline.
</jats:p>