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Medientyp:
E-Artikel
Titel:
A novel locus conferring fluoroquinolone resistance in Staphylococcus aureus
Beteiligte:
Trucksis, M;
Wolfson, J S;
Hooper, D C
Erschienen:
American Society for Microbiology, 1991
Erschienen in:
Journal of Bacteriology, 173 (1991) 18, Seite 5854-5860
Sprache:
Englisch
DOI:
10.1128/jb.173.18.5854-5860.1991
ISSN:
0021-9193;
1098-5530
Entstehung:
Anmerkungen:
Beschreibung:
Fluoroquinolones such as ciprofloxacin and ofloxacin are potent antimicrobial agents that antagonize the A subunit of DNA gyrase. We selected and mapped a novel fluoroquinolone resistance gene on the Staphylococcus aureus chromosome. Resistant mutants were selected with ciprofloxacin or ofloxacin and were uniformly localized to the A fragment of chromosomal DNA digested with SmaI and arrayed by pulsed-field gel electrophoresis. Several mutants (cfxB, ofxC) were genetically mapped between the thr and trp loci in the A fragment. A majority of A fragment fluoroquinolone resistance mutations were associated with reduced susceptibility to novobiocin, an antagonist of the B subunit of DNA gyrase. Two genes previously associated with fluoroquinolone resistance, the gyrA gene of DNA gyrase and the norA gene (associated with decreased drug accumulation), were localized to the G and D fragments, respectively. Thus, the fluoroquinolone resistance mutations in the A fragment are distinct from previously identified fluoroquinolone resistance mutations in gyrA and norA. Whether mutations in the A fragment after a second topoisomerase or another gene controlling supercoiling or affect drug permeation is unknown.