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Medientyp:
E-Artikel
Titel:
An Operon for a Putative ATP-Binding Cassette Transport System Involved in Acetoin Utilization of Bacillus subtilis
Beteiligte:
Yoshida, Ken-Ichi;
Fujita, Yasutaro;
Ehrlich, S. Dusko
Erschienen:
American Society for Microbiology, 2000
Erschienen in:Journal of Bacteriology
Sprache:
Englisch
DOI:
10.1128/jb.182.19.5454-5461.2000
ISSN:
0021-9193;
1098-5530
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>ABSTRACT</jats:title>
<jats:p>
The
<jats:italic>ytrABCDEF</jats:italic>
operon of
<jats:italic>Bacillus subtilis</jats:italic>
was deduced to encode a putative ATP-binding cassette (ABC) transport system. YtrB and YtrE could be the ABC subunits, and YtrC and YtrD are highly hydrophobic and could form a channel through the cell membrane, while YtrF could be a periplasmic lipoprotein for substrate binding. Expression of the operon was examined in cells grown in a minimal medium. The results indicate that the expression was induced only early in the stationary phase. The six
<jats:italic>ytr</jats:italic>
genes form a single operon, transcribed from a putative ς
<jats:sup>A</jats:sup>
-dependent promoter present upstream of
<jats:italic>ytrA</jats:italic>
. YtrA, which possesses a helix-turn-helix motif of the GntR family, acts probably as a repressor and regulates its own transcription. Inactivation of the operon led to a decrease in maximum cell yield and less-efficient sporulation, suggesting its involvement in the growth in stationary phase and sporulation. It is known that
<jats:italic>B. subtilis</jats:italic>
produces acetoin as an external carbon storage compound and then reuses it later during stationary phase and sporulation. When either the entire
<jats:italic>ytr</jats:italic>
operon or its last gene,
<jats:italic>ytrF</jats:italic>
, was inactivated, the production of acetoin was not affected, but the reuse of acetoin became less efficient. We suggest that the Ytr transport system plays a role in acetoin utilization during stationary phase and sporulation.
</jats:p>