Erschienen:
American Society for Microbiology, 2002
Erschienen in:Journal of Bacteriology
Sprache:
Englisch
DOI:
10.1128/jb.184.2.350-362.2002
ISSN:
0021-9193;
1098-5530
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>ABSTRACT</jats:title>
<jats:p>
Two-component systems are signal transduction systems which enable bacteria to regulate cellular functions in response to changing environmental conditions. In most cases regulation is accomplished on the transcriptional level by a response regulator protein, which, according to the phosphorylation state of its receiver domain, displays different affinities for its target promoters. Here we describe identification of genes regulated by the two-component system HP166-HP165 of
<jats:italic>Helicobacter pylori</jats:italic>
and characterization of the corresponding target promoters. We demonstrated that expression of the HP166-HP165 two-component system is negatively autoregulated under conditions favoring autophosphorylation of the histidine kinase. Furthermore, we found that response regulator HP166 activates transcription of genes encoding a protein family with an unknown function present in
<jats:italic>H. pylori</jats:italic>
26695, as well as an operon composed of five
<jats:italic>H. pylori-</jats:italic>
specific genes. While open reading frame HP166 is an essential gene, the target genes of the response regulator are not required for growth under in vitro culture conditions.
</jats:p>