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Medientyp:
E-Artikel
Titel:
Hepatitis C Virus RNA Replication Requires a Conserved Structural Motif within the Transmembrane Domain of the NS5B RNA-Dependent RNA Polymerase
Erschienen:
American Society for Microbiology, 2010
Erschienen in:
Journal of Virology, 84 (2010) 21, Seite 11580-11584
Sprache:
Englisch
DOI:
10.1128/jvi.01519-10
ISSN:
0022-538X;
1098-5514
Entstehung:
Anmerkungen:
Beschreibung:
ABSTRACT Hepatitis C virus (HCV) nonstructural protein 5B (NS5B), the viral RNA-dependent RNA polymerase (RdRp), is a tail-anchored protein with a highly conserved C-terminal transmembrane domain (TMD) that is required for the assembly of a functional replication complex. Here, we report that the TMD of the HCV RdRp can be functionally replaced by a newly identified analogous membrane anchor of the GB virus B (GBV-B) NS5B RdRp. Replicons with a chimeric RdRp consisting of the HCV catalytic domain and the GBV-B membrane anchor replicated with reduced efficiency. Compensatory amino acid changes at defined positions within the TMD improved the replication efficiency of these chimeras. These observations highlight a conserved structural motif within the TMD of the HCV NS5B RdRp that is required for RNA replication.