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Medientyp:
E-Artikel
Titel:
Growth Suppression Induced by Downregulation of E6-AP Expression in Human Papillomavirus-Positive Cancer Cell Lines Depends on p53
Beteiligte:
Hengstermann, Arnd;
D'silva, Michael A.;
Kuballa, Petric;
Butz, Karin;
Hoppe-Seyler, Felix;
Scheffner, Martin
Erschienen:
American Society for Microbiology, 2005
Erschienen in:
Journal of Virology, 79 (2005) 14, Seite 9296-9300
Sprache:
Englisch
DOI:
10.1128/jvi.79.14.9296-9300.2005
ISSN:
0022-538X;
1098-5514
Entstehung:
Anmerkungen:
Beschreibung:
ABSTRACT The ubiquitin-protein ligase E6-AP is utilized by the E6 oncoprotein of human papillomaviruses (HPVs) associated with cervical cancer to target the tumor suppressor p53 for degradation. Here, we report that downregulation of E6-AP expression by RNA interference results in both the accumulation of p53 and growth suppression of the HPV-positive cervical cancer cell lines HeLa and SiHa. In addition, HeLa cells, in which p53 expression was suppressed by RNA interference, are significantly less sensitive to the downregulation of E6-AP expression with respect to growth suppression than parental HeLa cells. These data indicate that the anti-growth-suppressive properties of E6-AP in HPV-positive cells depend on its ability to induce p53 degradation.