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Medientyp:
E-Artikel
Titel:
Proteomics of Protein Secretion by Bacillus subtilis : Separating the “Secrets” of the Secretome
Beteiligte:
Tjalsma, Harold;
Antelmann, Haike;
Jongbloed, Jan D.H.;
Braun, Peter G.;
Darmon, Elise;
Dorenbos, Ronald;
Dubois, Jean-Yves F.;
Westers, Helga;
Zanen, Geeske;
Quax, Wim J.;
Kuipers, Oscar P.;
Bron, Sierd;
Hecker, Michael;
van Dijl, Jan Maarten
Erschienen:
American Society for Microbiology, 2004
Erschienen in:
Microbiology and Molecular Biology Reviews, 68 (2004) 2, Seite 207-233
Sprache:
Englisch
DOI:
10.1128/mmbr.68.2.207-233.2004
ISSN:
1092-2172;
1098-5557
Entstehung:
Anmerkungen:
Beschreibung:
SUMMARY Secretory proteins perform a variety of important“remote-control” functions for bacterial survival inthe environment. The availability of complete genome sequences hasallowed us to make predictions about the composition of bacterialmachinery for protein secretion as well as the extracellular complementof bacterial proteomes. Recently, the power of proteomics wassuccessfully employed to evaluate genome-based models of theseso-called secretomes. Progress in this field is well illustrated by theproteomic analysis of protein secretion by the gram-positive bacterium Bacillus subtilis , for which ∼90 extracellularproteins were identified. Analysis of these proteins disclosed various“secrets of the secretome,” such as the residence ofcytoplasmic and predicted cell envelope proteins in the extracellularproteome. This showed that genome-based predictions reflect only∼50% of the actual composition of the extracellularproteome of B. subtilis . Importantly, proteomics allowed thefirst verification of the impact of individual secretion machinerycomponents on the total flow of proteins from the cytoplasm to theextracellular environment. In conclusion, proteomics has yielded avariety of novel leads for the analysis of protein traffic in B.subtilis and other gram-positive bacteria. Ultimately, such leadswill serve to increase our understanding of virulence factor biogenesisin gram-positive pathogens, which is likely to be of high medicalrelevance.