Conditional Hfq Association with Small Noncoding RNAs in Pseudomonas aeruginosa Revealed through Comparative UV Cross-Linking Immunoprecipitation Followed by High-Throughput Sequencing
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Medientyp:
E-Artikel
Titel:
Conditional Hfq Association with Small Noncoding RNAs in Pseudomonas aeruginosa Revealed through Comparative UV Cross-Linking Immunoprecipitation Followed by High-Throughput Sequencing
Erschienen:
American Society for Microbiology, 2019
Erschienen in:mSystems
Sprache:
Englisch
DOI:
10.1128/msystems.00590-19
ISSN:
2379-5077
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>
The Gram-negative bacterium
<jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content>
is ubiquitously distributed in diverse environments and can cause severe biofilm-related infections in at-risk individuals. Although the presence of a large number of putative sRNAs and widely conserved RNA chaperones in this bacterium implies the importance of posttranscriptional regulatory networks for environmental fluctuations, limited information is available regarding the global role of RNA chaperones such as Hfq in the
<jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content>
transcriptome, especially under different environmental conditions. Here, we characterize Hfq-dependent differences in gene expression and biological processes in two physiological states: the planktonic and biofilm forms. A combinatorial comparative CLIP-seq and total RNA-seq approach uncovered condition-dependent association of RNAs with Hfq
<jats:italic>in vivo</jats:italic>
and expands the potential direct regulatory targets of Hfq in the
<jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content>
transcriptome.
</jats:p>