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Moraleda, Cinta; Domínguez-Rodríguez, Sara; Mesa, Juan Miguel; García-Sánchez, Paula; de la Serna, María; Alonso-Cadenas, José Antonio; Bermejo, Amanda; Sabrido, Gema; Martínez-Campos, Leticia; González-Posada, Aránzazu Flavia; Illán-Ramos, Marta; Cobos-Carrascosa, Elena; Ballesteros, Álvaro; Galán, Juan Carlos; Llorente, Francisco; Aguilera-Alonso, David; Jiménez, Ana Belén; Catalán, Pilar; Dahdouh, Elias; Navarro, Ignacio; Fernández-Garoz, Bárbara; Mendoza, Pablo; Pérez-Jorge, Concepción; Cabezas-Fernández, Teresa; [...]

Oral saliva swab reverse transcription PCR for Covid-19 in the paediatric population

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  • Medientyp: E-Artikel
  • Titel: Oral saliva swab reverse transcription PCR for Covid-19 in the paediatric population
  • Beteiligte: Moraleda, Cinta; Domínguez-Rodríguez, Sara; Mesa, Juan Miguel; García-Sánchez, Paula; de la Serna, María; Alonso-Cadenas, José Antonio; Bermejo, Amanda; Sabrido, Gema; Martínez-Campos, Leticia; González-Posada, Aránzazu Flavia; Illán-Ramos, Marta; Cobos-Carrascosa, Elena; Ballesteros, Álvaro; Galán, Juan Carlos; Llorente, Francisco; Aguilera-Alonso, David; Jiménez, Ana Belén; Catalán, Pilar; Dahdouh, Elias; Navarro, Ignacio; Fernández-Garoz, Bárbara; Mendoza, Pablo; Pérez-Jorge, Concepción; Cabezas-Fernández, Teresa; [...]
  • Erschienen: BMJ, 2022
  • Erschienen in: Archives of Disease in Childhood
  • Sprache: Englisch
  • DOI: 10.1136/archdischild-2021-323712
  • ISSN: 0003-9888; 1468-2044
  • Schlagwörter: Pediatrics, Perinatology and Child Health
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:sec><jats:title>Objectives</jats:title><jats:p>To evaluate the performance of oral saliva swab (OSS) reverse transcription PCR (RT-PCR) compared with RT-PCR and antigen rapid diagnostic test (Ag-RDT) on nasopharyngeal swabs (NPS) for SARS-CoV-2 in children.</jats:p></jats:sec><jats:sec><jats:title>Design</jats:title><jats:p>Cross-sectional multicentre diagnostic study.</jats:p></jats:sec><jats:sec><jats:title>Setting</jats:title><jats:p>Study nested in a prospective, observational cohort (EPICO-AEP) performed between February and March 2021 including 10 hospitals in Spain.</jats:p></jats:sec><jats:sec><jats:title>Patients</jats:title><jats:p>Children from 0 to 18 years with symptoms compatible with Covid-19 of ≤5 days of duration were included. Two NPS samples (Ag-RDT and RT-PCR) and one OSS sample for RT-PCR were collected.</jats:p></jats:sec><jats:sec><jats:title>Main outcome</jats:title><jats:p>Performance of Ag-RDT and RT-PCR on NPS and RT-PCR on OSS sample for SARS-CoV-2.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>1174 children were included, aged 3.8 years (IQR 1.7–9.0); 73/1174 (6.2%) patients tested positive by at least one of the techniques. Sensitivity and specificity of OSS RT-PCR were 72.1% (95% CI 59.7 to 81.9) and 99.6% (95% CI 99 to 99.9), respectively, versus 61.8% (95% CI 49.1 to 73) and 99.9% (95% CI 99.4 to 100) for the Ag-RDT. Kappa index was 0.79 (95% CI 0.72 to 0.88) for OSS RT-PCR and 0.74 (95% CI 0.65 to 0.84) for Ag-RDT versus NPS RT-PCR.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>RT-PCR on the OSS sample is an accurate option for SARS-CoV-2 testing in children. A less intrusive technique for younger patients, who usually are tested frequently, might increase the number of patients tested.</jats:p></jats:sec>