Schoenherr, Christina;
Serrels, Bryan;
Proby, Charlotte;
Cunningham, Debbie L.;
Findlay, Jane E.;
Baillie, George S.;
Heath, John K.;
Frame, Margaret C.
Beteiligte:
Schoenherr, Christina;
Serrels, Bryan;
Proby, Charlotte;
Cunningham, Debbie L.;
Findlay, Jane E.;
Baillie, George S.;
Heath, John K.;
Frame, Margaret C.
Erschienen:
The Company of Biologists, 2014
Erschienen in:
Journal of Cell Science (2014)
Sprache:
Englisch
DOI:
10.1242/jcs.157560
ISSN:
1477-9137;
0021-9533
Entstehung:
Anmerkungen:
Beschreibung:
Eps8 is an actin regulatory scaffold protein increased in Squamous Cell Carcinoma (SCC) cells. It forms a complex with both Focal Adhesion Kinase (FAK) and c-Src in SCC cells derived from the DMBA/TPA model of skin carcinogenesis. Here, we describe two new roles for Eps8. Firstly, it controls the spatial distribution of active c-Src in a FAK-dependent manner. Specifically, Eps8 participates in, and regulates, a biochemical complex with c-Src and drives c-Src's trafficking to autophagic structures that SCC cells use to cope with high levels of active c-Src when FAK is absent. Secondly, when FAK is expressed in SCC cells, so tethering active c-Src at focal adhesion complexes, Eps8 is also recruited to focal adhesions and is required for FAK-dependent polarization and invasion. Therefore, Eps8 is a critical mediator of Src/FAK-regulated processes; it participates in specific biochemical complexes and promotes actin re-arrangements that determine c-Src's spatial localization and Src/FAK functions in invasive migration.