Evaluation of a tandem gas chromatography/time-of-flight mass spectrometry metabolomics platform as a single method to investigate the effect of starvation on whole-animal metabolism in rainbow trout (Oncorhynchus mykiss)
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Evaluation of a tandem gas chromatography/time-of-flight mass spectrometry metabolomics platform as a single method to investigate the effect of starvation on whole-animal metabolism in rainbow trout (Oncorhynchus mykiss)
Beteiligte:
Baumgarner, Bradley L.;
Cooper, Bruce R.
Beschreibung:
<jats:title>SUMMARY</jats:title>
<jats:p>This study was conducted to evaluate the use of a two-dimensional gas chromatography/time-of-flight mass spectrometry (GC×GC/TOF-MS) metabolomic platform to comprehensively analyze the effect of starvation on whole-animal metabolism in rainbow trout (Oncorhynchus mykiss). Trout were either fed a commercial diet at 2% body mass twice daily or starved for 4 weeks. Metabolomic analysis was conducted on serum, liver and muscle tissue from each fish. Database searching and statistical analysis revealed that concentrations of more than 50 positively identified molecules changed significantly (P&lt;0.05) as a result of starvation. Our results indicate that starving rainbow trout for 4 weeks promotes increased utilization of select tissue fatty acids in liver and muscle. However, starvation did not significantly affect protein catabolism in peripheral tissues, as indicated by reductions in the level of serum amino acids in starved fish. In contrast, starvation appears to promote protein catabolism in liver as the level of methionine, proline and lysine metabolite 2-piperidine carboxylic acid increased significantly. Also, starvation resulted in significant changes in the level of numerous xenobiotics that could indicate the origin of particular feed ingredients and selective retention of these molecules in tissues. We suggest that metabolomic analysis using GC×GC/TOF-MS is an effective tool in studying whole-animal metabolism and the fate of important xenobiotic compounds in rainbow trout as numerous polar and non-polar metabolites were rapidly and accurately profiled using a single method.</jats:p>