> Detailanzeige

Garofalo, Eugenio; Biamonte, Flavia; Palmieri, Camillo; Battaglia, Anna Martina; Sacco, Alessandro; Biamonte, Eugenio; Neri, Giuseppe; Antico, Giulio Cesare; Mancuso, Serafina; Foti, Giuseppe; Torti, Carlo; Costanzo, Francesco Saverio; Longhini, Federico; Bruni, Andrea

Severe and mild-moderate SARS-CoV-2 vaccinated patients show different frequencies of IFNγ-releasing cells: An exploratory study

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Severe and mild-moderate SARS-CoV-2 vaccinated patients show different frequencies of IFNγ-releasing cells: An exploratory study
  • Beteiligte: Garofalo, Eugenio; Biamonte, Flavia; Palmieri, Camillo; Battaglia, Anna Martina; Sacco, Alessandro; Biamonte, Eugenio; Neri, Giuseppe; Antico, Giulio Cesare; Mancuso, Serafina; Foti, Giuseppe; Torti, Carlo; Costanzo, Francesco Saverio; Longhini, Federico; Bruni, Andrea
  • Erschienen: Public Library of Science (PLoS), 2023
  • Erschienen in: PLOS ONE
  • Sprache: Englisch
  • DOI: 10.1371/journal.pone.0281444
  • ISSN: 1932-6203
  • Schlagwörter: Multidisciplinary
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:sec id="sec001"> <jats:title>Background</jats:title> <jats:p>Despite an apparent effective vaccination, some patients are admitted to the hospital after SARS-CoV-2 infection. The role of adaptive immunity in COVID-19 is growing; nonetheless, differences in the spike-specific immune responses between patients requiring or not hospitalization for SARS-CoV-2 infection remains to be evaluated. In this study, we aim to evaluate the spike-specific immune response in patients with mild-moderate or severeSARS-CoV-2 infection, after breakthrough infection following two doses of BNT162b2 mRNA vaccine.</jats:p> </jats:sec> <jats:sec id="sec002"> <jats:title>Methods</jats:title> <jats:p>We included three cohorts of 15 cases which received the two BNT162b2 vaccine doses in previous 4 to 7 months: 1) patients with severe COVID-19; 2) patients with mild-moderate COVID-19 and 3) vaccinated individuals with a negative SARS-CoV-2 molecular pharyngeal swab (healthy subjects). Anti-S1 and anti-S2 specific SARS-CoV-2 IgM and IgG titers were measured through a chemiluminescence immunoassay technology. In addition, the frequencies of IFNγ-releasing cells were measured by ELISpot.</jats:p> </jats:sec> <jats:sec id="sec003"> <jats:title>Results</jats:title> <jats:p>The spike-specific IFNγ-releasing cells were significantly lower in severe patients (8 [0; 26] s.f.c.×10<jats:sup>6</jats:sup>), as compared to mild-moderate patients (135 [64; 159] s.f.c.×10<jats:sup>6</jats:sup>; p&lt;0.001) and healthy subjects (103 [50; 188] s.f.c.×10<jats:sup>6</jats:sup>; p&lt;0.001). The anti-Spike protein IgG levels were similar among the three cohorts of cases (p = 0.098). All cases had an IgM titer below the analytic sensitivity of the test. The Receiver Operating Curve analysis indicated the rate of spike-specific IFNγ-releasing cells can discriminate correctly severe COVID-19 and mild-moderate patients (AUC: 0.9289; 95%CI: 0.8376–1.000; p&lt; 0.0001), with a diagnostic specificity of 100% for s.f.c. &gt; 81.2 x 10<jats:sup>6</jats:sup>.</jats:p> </jats:sec> <jats:sec id="sec004"> <jats:title>Conclusions</jats:title> <jats:p>2-doses vaccinated patients requiring hospitalization for severe COVID-19 show a cellular-mediated immune response lower than mild-moderate or healthy subjects, despite similar antibody titers.</jats:p> </jats:sec>
  • Zugangsstatus: Freier Zugang