Trägergebundene Serotoninderivate und Affinitätschromatographie synaptischer Membranproteine / Resin Linked Derivatives of Serotonin and Affinity Chromatography of Proteins Isolated from Synaptic Membranes
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Medientyp:
E-Artikel
Titel:
Trägergebundene Serotoninderivate und Affinitätschromatographie synaptischer Membranproteine / Resin Linked Derivatives of Serotonin and Affinity Chromatography of Proteins Isolated from Synaptic Membranes
Beteiligte:
Seela, Frank;
Wesemann, Wolfgang
Erschienen:
Walter de Gruyter GmbH, 1974
Erschienen in:
Zeitschrift für Naturforschung C, 29 (1974) 5-6, Seite 248-256
Beschreibung:
An affinity resin for the isolation of the serotonin receptor from nerve ending membranes was prepared by coupling tryptamine, 5-methoxy-tryptamine or serotonin to CNBr-activated Sepharose 4 B. The tryptamine derivatives, N-6-(aminohexyl)-tryptamines, were obtained by subsequent con densation of the corresponding indole derivatives with oxalyl chloride and t-aminocaproamide and reduction with LiAlH4. The 6-aminohexyl-group was used as spacer to link covalently the primary amino-group of tryptamine to agarose. - An extract of membrane proteins isolated from rat brain was resolved on this affinity resin into two fractions as compared with only one fraction using unsubstituted Sepharose 4 B.