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Heidrich, Jens P.; Niemeier, Anja; Seyfarth, Michael

Continuous Analysis in Extracorporeally Circulating Blood - A Rat Model Applying Flow-Through Ion-Selective Electrodes for the Measurement of Ca2+, K+, Na+ and pH

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  • Medientyp: E-Artikel
  • Titel: Continuous Analysis in Extracorporeally Circulating Blood - A Rat Model Applying Flow-Through Ion-Selective Electrodes for the Measurement of Ca2+, K+, Na+ and pH
  • Beteiligte: Heidrich, Jens P.; Niemeier, Anja; Seyfarth, Michael
  • Erschienen: Walter de Gruyter GmbH, 1998
  • Erschienen in: cclm, 36 (1998) 11, Seite 847-854
  • Sprache: Englisch
  • DOI: 10.1515/cclm.1998.149
  • ISSN: 1434-6621; 1437-4331
  • Schlagwörter: Biochemistry (medical) ; Clinical Biochemistry ; General Medicine
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: Abstract A rat model is introduced which enables investigations in anticoagulated blood with continuous measurements by a flow-through electrode system. In the present study, a potentiometric ion-selective electrode (ISE)-system was used for measuring Ca2+, K+, Na+ and pH in rats. The setup was adjusted to an extracorporeal blood-volume of 0.750 ml. This permits indirect measurements of the analytes via a dialysis membrane, with electrical separation of the ISE's and the animal. The flow-rates of blood and dialysis-solution were adjusted in such a way that water diffusing from the aqueous dialysis solution into the blood, across the dialysis membrane, does not alter the haematocrit. Polyethyleneglycol-hirudin was used for anticoagulation, since it was superior to heparin. The assembly enables continuous measurements in the living anaesthetized rat over a time period of at least 3 hours.