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Medientyp:
E-Artikel
Titel:
Endophytic bacterial strain,Brevibacillus brevis-mediated green synthesis of copper oxide nanoparticles, characterization, antifungal,in vitrocytotoxicity, and larvicidal activity
Beteiligte:
Fouda, Amr;
Hassan, Saad El-Din;
Eid, Ahmed M.;
Awad, Mohamed A.;
Althumayri, Khalid;
Badr, Naglaa Fathi;
Hamza, Mohammed F.
Erschienen:
Walter de Gruyter GmbH, 2022
Erschienen in:Green Processing and Synthesis
Sprache:
Englisch
DOI:
10.1515/gps-2022-0080
ISSN:
2191-9550
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Abstract</jats:title><jats:p>The biomass filtrate containing various metabolites of endophytic bacterial strain,<jats:italic>Brevibacillus brevis</jats:italic>PI-5 was used as a biocatalyst for reducing and stabilizing copper oxide nanoparticles (CuO-NPs). UV-Vis spectroscopy, Fourier transform infrared, transmission electron microscopy, scanning electron microscopy with energy-dispersive X-ray (SEM-EDX), X-ray diffraction, and X-ray photoelectron spectroscopy were used for CuO-NPs characterization. A spherical, well-dispersed, and crystallographic structure with sizes of 2–28 nm was formed. The SEM-EDX confirmed the presence of Cu and O with weight percentages of 27.62% and 48.88%, respectively. The biological activities including antifungal, anticancer, and larvicidal of synthesized CuO-NPs were assessed using the fungal radial growth inhibition, MTT assay method, and mortality percentages, respectively. The obtained data showed that the CuO-NPs exhibit high activity in a dose-dependent manner. The growth of three phytopathogenic fungi,<jats:italic>Fusarium oxysporum, Alternaria alternata</jats:italic>, and<jats:italic>Aspergillus niger</jats:italic>was decreased by percentages of 64.5% ± 4.1%, 62.9% ± 0.3%, and 70.2% ± 2.3%, respectively at 300 µg·mL<jats:sup>–1</jats:sup>. Also, various clinical<jats:italic>Candida</jats:italic>spp. were successfully inhibited with varied zones of inhibition and minimum inhibitory concentration values in ranges of 6.25–50 µg·mL<jats:sup>–1</jats:sup>. The<jats:italic>in vitro</jats:italic>cytotoxicity exhibits target-orientation to breast cancer cells (T47D) at low concentration compared to normal cells (HFB4) with IC<jats:sub>50</jats:sub>values of 122.3 ± 5.4 and 229.9 ± 5.7 μg·mL<jats:sup>–1</jats:sup>, respectively. The mortality percentages of I, II, III, and IV instar larvae of<jats:italic>Culex antennatus</jats:italic>were 60% ± 1.4%, 43.1% ± 1.1%, 36.2% ± 1%, and 32.1% ± 0.9%, at 10 mg·L<jats:sup>–1</jats:sup>and increased to 86.9% ± 2.1%, 68.1% ± 1.7%, 64.4% ± 1.9%, and 53.1% ± 1.4% at 50 mg·L<jats:sup>–1</jats:sup>, respectively.</jats:p>