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Lima, Dânya Bandeira; Mello, Clarissa Perdigão; Bandeira, Izabel Cristina Justino; Pessoa Bezerra de Menezes, Ramon Róseo Paula; Sampaio, Tiago Lima; Falcão, Cláudio Borges; Morlighem, Jean-Étienne R.L.; Rádis-Baptista, Gandhi; Martins, Alice Maria Costa

The dinoponeratoxin peptides from the giant ant Dinoponera quadriceps display in vitro antitrypanosomal activity

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  • Medientyp: E-Artikel
  • Titel: The dinoponeratoxin peptides from the giant ant Dinoponera quadriceps display in vitro antitrypanosomal activity
  • Beteiligte: Lima, Dânya Bandeira; Mello, Clarissa Perdigão; Bandeira, Izabel Cristina Justino; Pessoa Bezerra de Menezes, Ramon Róseo Paula; Sampaio, Tiago Lima; Falcão, Cláudio Borges; Morlighem, Jean-Étienne R.L.; Rádis-Baptista, Gandhi; Martins, Alice Maria Costa
  • Erschienen: Walter de Gruyter GmbH, 2018
  • Erschienen in: Biological Chemistry, 399 (2018) 2, Seite 187-196
  • Sprache: Englisch
  • DOI: 10.1515/hsz-2017-0198
  • ISSN: 1437-4315; 1431-6730
  • Schlagwörter: Clinical Biochemistry ; Molecular Biology ; Biochemistry
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title> <jats:p>The crude venom of the giant ant <jats:italic>Dinoponera quadriceps</jats:italic> is a cocktail of polypeptides and organic compounds that shows antiparasitic effects against <jats:italic>Trypanosoma cruzi</jats:italic>, the causative agent of Chagas disease. In order to investigate the venom-derived components responsible for such antitrypanosomal activity, four dinoponeratoxins (DnTxs) were identified, namely M-PONTX-Dq3a, -Dq3b, -Dq3c and -Dq4e, that are diverse in size, net charge, hydrophobicity and propensity to interact with eukaryote cell membranes. These peptides were tested against epimastigote, trypomastigote and amastigote forms of benznidazole (Bz)-resistant Y strain of <jats:italic>T. cruzi</jats:italic> and in mammalian host cells. The M-PONTX-Dq3a and -Dq4e inhibited all developmental forms of <jats:italic>T. cruzi</jats:italic>, including amastigotes, the responsible form for the maintenance of infection on chronic phase of the disease. The M-PONTX-Dq3a showed the highest selectivity index (SI) (80) and caused morphological alterations in <jats:italic>T. cruzi</jats:italic>, as observed by scanning electron microscopy (SEM), and induced cell death through necrosis, as seen by multiparametric flow cytometry analysis with specific biochemical markers. Altogether, the <jats:italic>D. quadriceps</jats:italic> venom appears as a source for the prospection of trypanocidal peptides and the M-PONTX-Dq3a arises as a candidate among the dinoponeratoxin-related peptides in the development of compounds against Chagas disease.</jats:p>