Erschienen in:
Biological Chemistry, 400 (2019) 3, Seite 289-297
Sprache:
Englisch
DOI:
10.1515/hsz-2018-0358
ISSN:
1437-4315;
1431-6730
Entstehung:
Anmerkungen:
Beschreibung:
Abstract Site-specific bioconjugation strategies offer many possibilities for directed protein modifications. Among the various enzyme-based conjugation protocols, formylglycine-generating enzymes allow to posttranslationally introduce the amino acid Cα-formylglycine (FGly) into recombinant proteins, starting from cysteine or serine residues within distinct consensus motifs. The aldehyde-bearing FGly-residue displays orthogonal reactivity to all other natural amino acids and can, therefore, be used for site-specific labeling reactions on protein scaffolds. In this review, the state of research on catalytic mechanisms and consensus motifs of different formylglycine-generating enzymes, as well as labeling strategies and applications of FGly-based bioconjugations are presented.