Hamid Tahtamouni, Lubna;
Nael Hamdan, Mahmoud;
Ali Al-Mazaydeh, Zainab;
Mahmoud Bawadi, Randa;
Sobhi Rammaha, Majdoleen;
Mohammad Zghoul, Ahmad;
Ahmad Ahram, Mamoun;
Refat Yasin, Salem
Alu-repeat polymorphism in the tissue plasminogen activator (t-PA) gene, seminal t-PA concentration, and male fertility impairment: A case-control study
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Medientyp:
E-Artikel
Titel:
Alu-repeat polymorphism in the tissue plasminogen activator (t-PA) gene, seminal t-PA concentration, and male fertility impairment: A case-control study
Beteiligte:
Hamid Tahtamouni, Lubna;
Nael Hamdan, Mahmoud;
Ali Al-Mazaydeh, Zainab;
Mahmoud Bawadi, Randa;
Sobhi Rammaha, Majdoleen;
Mohammad Zghoul, Ahmad;
Ahmad Ahram, Mamoun;
Refat Yasin, Salem
Erschienen:
Knowledge E, 2020
Erschienen in:International Journal of Reproductive BioMedicine (IJRM)
Beschreibung:
<jats:p>Background: Tissue plasminogen activator (t-PA) is a protein involved in the fibrinolytic system that catalyzes the conversion of plasminogen into the active plasmin. The activity of t-PA is controlled by plasminogen activator inhibitor-1. t-PA has crucial functions during spermatogenesis. One polymorphism was reported for t-PA gene, either the presence of a 300-bp Alu-repeat (Alu+) or its absence (Alu−).
Objective: The current work aimed at studying the association between Alu polymorphism in the t-PA gene and male infertility.
Materials and Methods: Using polymerase chain reaction on genomic DNA isolated from the blood of 79 participants, a region polymorphic for Alu element insertion in t-PA gene was amplified. In addition, total t-PA concentration, plasminogen activator inhibitor-1/t-PA complex concentration, and t-PA activity in seminal plasma were measured by enzyme-linked immunosorbent assay.
Results: The results indicate that the percentage of infertile participants (n = 50) who were homozygous for t-PA Alu insertion (Alu+/+), heterozygous Alu+/− or homozygous for t-PA Alu deletion (Alu−/−) did not change significantly (p = 0.43, 0.81, and 0.85, respectively) when compared with the control participants (n = 29). On the other hand, a significant decrease (p = 0.0001) of t-PA total concentration in seminal plasma was observed in the infertile group in comparison with the control group. However, the results indicate that there is no association between the t-PA Alu different genotypes and the total t-PA seminal concentration in the infertile group when compared to the control group (p = 0.63).
Conclusion: Data obtained from the current study does not support an association between t-PA Alu polymorphism and t-PA seminal concentration or male infertility.
Key words: Alu element, Male infertility, Semen, Spermatogenesis, t-PA.</jats:p>