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Michalková, R.; Šiviková, K.; Galdíková, M.

Analysis of Sister Chromatid Exchanges and Proliferation of Human Peripheral Blood Lymphocytes Exposed to Epoxiconazole

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  • Medientyp: E-Artikel
  • Titel: Analysis of Sister Chromatid Exchanges and Proliferation of Human Peripheral Blood Lymphocytes Exposed to Epoxiconazole
  • Beteiligte: Michalková, R.; Šiviková, K.; Galdíková, M.
  • Erschienen: Walter de Gruyter GmbH, 2018
  • Erschienen in: Folia Veterinaria
  • Sprache: Englisch
  • DOI: 10.2478/fv-2018-0026
  • ISSN: 2453-7837
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title> <jats:p>The potential genotoxic/cytotoxic effect of epoxiconazole was evaluated by means of sister chromatid exchanges (SCE) following the 24 and 48 h <jats:italic>in vitro</jats:italic> exposure of human peripheral blood lymphocytes to epoxiconazole at concentrations of: 5, 10, 25, 50 and 100 μg. ml<jats:sup>–1</jats:sup>. Dimethyl sulphoxide (DMSO), used as an epoxiconazole solvent, was used as a negative control and mitomycine (MMC) as a positive control. After the 24-hour exposure, we failed to observe a significant increase in SCE frequencies in comparison with the negative control, however, the concentrations of 10—100 μg.ml<jats:sup>–1</jats:sup> caused a significant decrease in the proliferation index (PI; P &lt; 0.001). Also, the 48-hour exposure produced no significant alterations in the SCE frequencies in comparison with the control. At epoxiconazole concentrations ranging from 10 to 50 μg.ml<jats:sup>–1</jats:sup> we recorded a moderate to strong, dose-dependent inhibition of PI (P &lt; 0.05; P &lt; 0.01; P &lt; 0.001), while at the highest dose (100 μg.ml<jats:sup>–1</jats:sup>) the reduction in PI compared to the control was less pronounced (P &lt; 0.05). The reduction in PI at the concentration range of 10—100 μg.ml<jats:sup>–1</jats:sup> depended on the number of cells in the M<jats:sub>1</jats:sub>, M<jats:sub>2</jats:sub> and M<jats:sub>3</jats:sub> phases of the cell cycle per total number of 100 evaluated metaphases. Our results indicated a significant cytotoxic or cytostatic effect on human peripheral blood lymphocytes.</jats:p>