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Crozet, Flora; Letort, Gaëlle; Bulteau, Rose; Da Silva, Christelle; Eichmuller, Adrien; Tortorelli, Anna Francesca; Blévinal, Joséphine; Belle, Morgane; Dumont, Julien; Piolot, Tristan; Dauphin, Aurélien; Coulpier, Fanny; Chédotal, Alain; Maître, Jean-Léon; Verlhac, Marie-Hélène; Clarke, Hugh J; Terret, Marie-Emilie

Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes

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  • Medientyp: E-Artikel
  • Titel: Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes
  • Beteiligte: Crozet, Flora; Letort, Gaëlle; Bulteau, Rose; Da Silva, Christelle; Eichmuller, Adrien; Tortorelli, Anna Francesca; Blévinal, Joséphine; Belle, Morgane; Dumont, Julien; Piolot, Tristan; Dauphin, Aurélien; Coulpier, Fanny; Chédotal, Alain; Maître, Jean-Léon; Verlhac, Marie-Hélène; Clarke, Hugh J; Terret, Marie-Emilie
  • Erschienen: Life Science Alliance, LLC, 2023
  • Erschienen in: Life Science Alliance, 6 (2023) 6, Seite e202301963
  • Sprache: Englisch
  • DOI: 10.26508/lsa.202301963
  • ISSN: 2575-1077
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: The oocyte must grow and mature before fertilization, thanks to a close dialogue with the somatic cells that surround it. Part of this communication is through filopodia-like protrusions, called transzonal projections (TZPs), sent by the somatic cells to the oocyte membrane. To investigate the contribution of TZPs to oocyte quality, we impaired their structure by generating a full knockout mouse of the TZP structural component myosin-X (MYO10). Using spinning disk and super-resolution microscopy combined with a machine-learning approach to phenotype oocyte morphology, we show that the lack ofMyo10decreases TZP density during oocyte growth. Reduction in TZPs does not prevent oocyte growth but impairs oocyte-matrix integrity. Importantly, we reveal by transcriptomic analysis that gene expression is altered in TZP-deprived oocytes and that oocyte maturation and subsequent early embryonic development are partially affected, effectively reducing mouse fertility. We propose that TZPs play a role in the structural integrity of the germline–somatic complex, which is essential for regulating gene expression in the oocyte and thus its developmental potential.
  • Zugangsstatus: Freier Zugang