> Detailanzeige

Sung, Abby; Bailey, Adam L.; Stewart, Henry B.; McDonald, David; Wallace, Meghan A.; Peacock, Kate; Miller, Candace; Reske, Kimberly A.; O’Neil, Caroline A.; Fraser, Victoria J.; Diamond, Michael S.; Burnham, Carey-Ann D.; Babcock, Hilary M.; Kwon, Jennie H.

Isolation of SARS-CoV-2 in Viral Cell Culture in Immunocompromised Patients With Persistently Positive RT-PCR Results

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Isolation of SARS-CoV-2 in Viral Cell Culture in Immunocompromised Patients With Persistently Positive RT-PCR Results
  • Beteiligte: Sung, Abby; Bailey, Adam L.; Stewart, Henry B.; McDonald, David; Wallace, Meghan A.; Peacock, Kate; Miller, Candace; Reske, Kimberly A.; O’Neil, Caroline A.; Fraser, Victoria J.; Diamond, Michael S.; Burnham, Carey-Ann D.; Babcock, Hilary M.; Kwon, Jennie H.
  • Erschienen: Frontiers Media SA, 2022
  • Erschienen in: Frontiers in Cellular and Infection Microbiology
  • Sprache: Nicht zu entscheiden
  • DOI: 10.3389/fcimb.2022.804175
  • ISSN: 2235-2988
  • Schlagwörter: Infectious Diseases ; Microbiology (medical) ; Immunology ; Microbiology
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Immunocompromised adults can have prolonged acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive RT-PCR results, long after the initial diagnosis of coronavirus disease 2019 (COVID-19). This study aimed to determine if SARS-CoV-2 virus can be recovered in viral cell culture from immunocompromised adults with persistently positive SARS-CoV-2 RT-PCR tests. We obtained 20 remnant SARS-CoV-2 PCR positive nasopharyngeal swabs from 20 immunocompromised adults with a positive RT-PCR test ≥14 days after the initial positive test. The patients’ 2<jats:sup>nd</jats:sup> test samples underwent SARS-CoV-2 antigen testing, and culture with Vero-hACE2-TMPRSS2 cells. Viral RNA and cultivable virus were recovered from the cultured cells after qRT-PCR and plaque assays. Of 20 patients, 10 (50%) had a solid organ transplant and 5 (25%) had a hematologic malignancy. For most patients, RT-PCR Ct values increased over time. There were 2 patients with positive viral cell cultures; one patient had chronic lymphocytic leukemia treated with venetoclax and obinutuzumab who had a low viral titer of 27 PFU/mL. The second patient had marginal zone lymphoma treated with bendamustine and rituximab who had a high viral titer of 2 x 10<jats:sup>6</jats:sup> PFU/mL. Most samples collected ≥7 days after an initial positive SARS-CoV-2 RT-PCR had negative viral cell cultures. The 2 patients with positive viral cell cultures had hematologic malignancies treated with chemotherapy and B cell depleting therapy. One patient had a high concentration titer of cultivable virus. Further data are needed to determine risk factors for persistent viral shedding and methods to prevent SARS-CoV-2 transmission from immunocompromised hosts.</jats:p>
  • Zugangsstatus: Freier Zugang