Flores-Valdez, Mauricio;
Ares, Miguel A.;
Rosales-Reyes, Roberto;
Torres, Javier;
Girón, Jorge A.;
Weimer, Bart C.;
Mendez-Tenorio, Alfonso;
De la Cruz, Miguel A.
Whole Genome Sequencing of Pediatric Klebsiella pneumoniae Strains Reveals Important Insights Into Their Virulence-Associated Traits
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Medientyp:
E-Artikel
Titel:
Whole Genome Sequencing of Pediatric Klebsiella pneumoniae Strains Reveals Important Insights Into Their Virulence-Associated Traits
Beteiligte:
Flores-Valdez, Mauricio;
Ares, Miguel A.;
Rosales-Reyes, Roberto;
Torres, Javier;
Girón, Jorge A.;
Weimer, Bart C.;
Mendez-Tenorio, Alfonso;
De la Cruz, Miguel A.
Erschienen:
Frontiers Media SA, 2021
Erschienen in:Frontiers in Microbiology
Sprache:
Nicht zu entscheiden
DOI:
10.3389/fmicb.2021.711577
ISSN:
1664-302X
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p><jats:italic>Klebsiella pneumoniae</jats:italic> is recognized as a common cause of nosocomial infections and outbreaks causing pneumonia, septicemia, and urinary tract infections. This opportunistic bacterium shows an increasing acquisition of antibiotic-resistance genes, which complicates treatment of infections. Hence, fast reliable strain typing methods are paramount for the study of this opportunistic pathogen’s multi-drug resistance genetic profiles. In this study, thirty-eight strains of <jats:italic>K. pneumoniae</jats:italic> isolated from the blood of pediatric patients were characterized by whole-genome sequencing and genomic clustering methods. Genes encoding β-lactamase were found in all the bacterial isolates, among which the <jats:italic>bla</jats:italic><jats:sub>SHV</jats:sub> variant was the most prevalent (53%). Moreover, genes encoding virulence factors such as fimbriae, capsule, outer membrane proteins, T4SS and siderophores were investigated. Additionally, a multi-locus sequence typing (MLST) analysis revealed 24 distinct sequence types identified within the isolates, among which the most frequently represented were ST76 (16%) and ST70 (11%). Based on LPS structure, serotypes O1 and O3 were the most prevalent, accounting for approximately 63% of all infections. The virulence capsular types K10, K136, and K2 were present in 16, 13, and 8% of the isolates, respectively. Phylogenomic analysis based on virtual genome fingerprints correlated with the MLST data. The phylogenomic reconstruction also denoted association between strains with a higher abundance of virulence genes and virulent serotypes compared to strains that do not possess these traits. This study highlights the value of whole-genomic sequencing in the surveillance of virulence attributes among clinical <jats:italic>K. pneumoniae</jats:italic> strains.</jats:p>