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Imano, Sayaka; Fushimi, Mayuka; Camagna, Maurizio; Tsuyama-Koike, Akiko; Mori, Hitoshi; Ashida, Akira; Tanaka, Aiko; Sato, Ikuo; Chiba, Sotaro; Kawakita, Kazuhito; Ojika, Makoto; Takemoto, Daigo

AP2/ERF Transcription Factor NbERF-IX-33 Is Involved in the Regulation of Phytoalexin Production for the Resistance of Nicotiana benthamiana to Phytophthora infestans

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  • Medientyp: E-Artikel
  • Titel: AP2/ERF Transcription Factor NbERF-IX-33 Is Involved in the Regulation of Phytoalexin Production for the Resistance of Nicotiana benthamiana to Phytophthora infestans
  • Beteiligte: Imano, Sayaka; Fushimi, Mayuka; Camagna, Maurizio; Tsuyama-Koike, Akiko; Mori, Hitoshi; Ashida, Akira; Tanaka, Aiko; Sato, Ikuo; Chiba, Sotaro; Kawakita, Kazuhito; Ojika, Makoto; Takemoto, Daigo
  • Erschienen: Frontiers Media SA, 2022
  • Erschienen in: Frontiers in Plant Science
  • Sprache: Nicht zu entscheiden
  • DOI: 10.3389/fpls.2021.821574
  • ISSN: 1664-462X
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Plants recognize molecular patterns unique to a certain group of microbes to induce effective resistance mechanisms. Elicitins are secretory proteins produced by plant pathogenic oomycete genera including <jats:italic>Phytophthora</jats:italic> and <jats:italic>Pythium.</jats:italic> Treatment of INF1 (an elicitin produced by <jats:italic>P. infestans</jats:italic>) induces a series of defense responses in <jats:italic>Nicotiana</jats:italic> species, including reactive oxygen species (ROS) production, transient induction of ethylene production, hypersensitive cell death and accumulation of the sesquiterpenoid phytoalexin capsidiol. In this study, we analyzed the expression profiles of <jats:italic>N. benthamiana</jats:italic> genes after INF1 treatment by RNAseq analysis. Based on their expression patterns, <jats:italic>N. benthamiana</jats:italic> genes were categorized into 20 clusters and 4,761 (8.3%) out of 57,140 genes were assigned to the clusters for INF1-induced genes. All genes encoding enzymes dedicated to capsidiol production, 5-<jats:italic>epi</jats:italic>-aristolochene (EA) synthase (<jats:italic>NbEAS</jats:italic>, 10 copies) and EA dehydrogenase (<jats:italic>NbEAH</jats:italic>, 6 copies), and some genes for ethylene production, such as 1-aminocyclopropane 1-carboxylate (ACC) synthase (<jats:italic>NbACS</jats:italic>) and ACC oxidase (<jats:italic>NbACO</jats:italic>), were significantly upregulated by INF1 treatment. Analysis of <jats:italic>NbEAS1</jats:italic> and <jats:italic>NbEAS4</jats:italic> promoters revealed that AGACGCC (GCC box-like motif) is the essential cis-element required for INF1-induced expression of <jats:italic>NbEAS</jats:italic> genes. Given that the GCC box is known to be targeted by ERF (ethylene-responsive factor) transcription factors, we created a complete list of <jats:italic>N. benthamiana</jats:italic> genes encoding AP2/ERF family transcription factors, and identified 45 out of 337 <jats:italic>AP2/ERF</jats:italic> genes in the clusters for INF1-induced genes. Among INF1-induced <jats:italic>NbERF</jats:italic> genes, silencing of <jats:italic>NbERF-IX-33</jats:italic> compromised resistance against <jats:italic>P. infestans</jats:italic> and INF1-induced production of capsidiol. Recombinant NbERF-IX-33 protein can bind to the promoter sequence of <jats:italic>NbEAS4</jats:italic>, suggesting that NbERF-IX-33 is a transcription factor directly regulating the expression of genes for phytoalexin production.</jats:p>
  • Zugangsstatus: Freier Zugang