Holzhausen, Anja;
Stingl, Nora;
Rieth, Sophie;
Kühn, Christine;
Schubert, Hendrik;
Rensing, Stefan Andreas
Establishment and optimization of a new model organism to study early land plant evolution: Germination, cultivation and oospore variation of Chara braunii Gmelin, 1826
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Medientyp:
E-Artikel
Titel:
Establishment and optimization of a new model organism to study early land plant evolution: Germination, cultivation and oospore variation of Chara braunii Gmelin, 1826
Beteiligte:
Holzhausen, Anja;
Stingl, Nora;
Rieth, Sophie;
Kühn, Christine;
Schubert, Hendrik;
Rensing, Stefan Andreas
Erschienen:
Frontiers Media SA, 2022
Erschienen in:
Frontiers in Plant Science, 13 (2022)
Sprache:
Nicht zu entscheiden
DOI:
10.3389/fpls.2022.987741
ISSN:
1664-462X
Entstehung:
Anmerkungen:
Beschreibung:
For studying land plant evolution, the establishment and optimization of model organisms representing streptophytic algae, sister to land plants, is essential. Long-term cultivation experiments withChara brauniiS276 were performed over 8 years, since 4 years (Nov. 2018) under constant conditions. Additionally, short-term experiments for optimization of culture conditions were performed with three strains ofC. braunii(S276, NIES-1604 and Lausiger Teiche, LaT-2708). Germination success after application of sterilization agents, addition of gibberellic acid and under different incubation conditions with respect to pre-treatment, irradiance regime and substrate was investigated in order to develop protocols for generative cultivation of at least unialgal cultures. The resulting cultivation protocols forC. brauniiS276, allowing maintenance of vegetative as well as generative cultures are presented in detail, including protocols for germination induction and growth of sterilized and unsterilized oospores.