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Johansson, Emil; Kerkman, Priscilla F.; Scharf, Lydia; Lindman, Jacob; Szojka, Zsófia I.; Månsson, Fredrik; Biague, Antonio; Medstrand, Patrik; Norrgren, Hans; Buggert, Marcus; Karlsson, Annika C.; Forsell, Mattias N. E.; Esbjörnsson, Joakim; Jansson, Marianne

Hierarchical Clustering and Trajectory Analyses Reveal Viremia-Independent B-Cell Perturbations in HIV-2 Infection

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  • Medientyp: E-Artikel
  • Titel: Hierarchical Clustering and Trajectory Analyses Reveal Viremia-Independent B-Cell Perturbations in HIV-2 Infection
  • Beteiligte: Johansson, Emil; Kerkman, Priscilla F.; Scharf, Lydia; Lindman, Jacob; Szojka, Zsófia I.; Månsson, Fredrik; Biague, Antonio; Medstrand, Patrik; Norrgren, Hans; Buggert, Marcus; Karlsson, Annika C.; Forsell, Mattias N. E.; Esbjörnsson, Joakim; Jansson, Marianne
  • Erschienen: MDPI AG, 2022
  • Erschienen in: Cells
  • Sprache: Englisch
  • DOI: 10.3390/cells11193142
  • ISSN: 2073-4409
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Time to AIDS in HIV-2 infection is approximately twice as long compared to in HIV-1 infection. Despite reduced viremia, HIV-2-infected individuals display signs of chronic immune activation. In HIV-1-infected individuals, B-cell hyperactivation is driven by continuous antigen exposure. However, the contribution of viremia to B-cell perturbations in HIV-2-infected individuals remains largely unexplored. Here, we used polychromatic flow cytometry, consensus hierarchical clustering and pseudotime trajectory inference to characterize B-cells in HIV-1- or HIV-2-infected and in HIV seronegative individuals. We observed increased frequencies of clusters containing hyperactivated T-bethighCD95highCD27int and proliferating T-bet+CD95highCD27+CD71+ memory B-cells in viremic HIV-1 (p &lt; 0.001 and p &lt; 0.001, respectively), viremic HIV-2 (p &lt; 0.001 and p = 0.014, respectively) and in treatment-naïve aviremic HIV-2 (p = 0.004 and p = 0.020, respectively)-infected individuals, compared to seronegative individuals. In contrast, these expansions were not observed in successfully treated HIV-1-infected individuals. Finally, pseudotime trajectory inference showed that T-bet-expressing hyperactivated and proliferating memory B-cell populations were located at the terminal end of two trajectories, in both HIV-1 and HIV-2 infections. As the treatment-naïve aviremic HIV-2-infected individuals, but not the successfully ART-treated HIV-1-infected individuals, showed B-cell perturbations, our data suggest that aviremic HIV-2-infected individuals would also benefit from antiretroviral treatment.</jats:p>
  • Zugangsstatus: Freier Zugang