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Galang, Joelle Noriko; Shen, Yefeng; Koitzsch, Ulrike; Yu, Xiaojie; Eischeid-Scholz, Hannah; Bachurski, Daniel; Rau, Tilman T.; Neppl, Christina; Herling, Marco; Bulimaga, Bianca; Vasyutina, Elena; Schweiger, Michal R.; Büttner, Reinhard; Odenthal, Margarete; Anokhina, Maria M.

Vesicular Release and Uptake of Circular LSD1-RNAs from Non-Cancer and Cancer Lung Cells

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  • Medientyp: E-Artikel
  • Titel: Vesicular Release and Uptake of Circular LSD1-RNAs from Non-Cancer and Cancer Lung Cells
  • Beteiligte: Galang, Joelle Noriko; Shen, Yefeng; Koitzsch, Ulrike; Yu, Xiaojie; Eischeid-Scholz, Hannah; Bachurski, Daniel; Rau, Tilman T.; Neppl, Christina; Herling, Marco; Bulimaga, Bianca; Vasyutina, Elena; Schweiger, Michal R.; Büttner, Reinhard; Odenthal, Margarete; Anokhina, Maria M.
  • Erschienen: MDPI AG, 2023
  • Erschienen in: International Journal of Molecular Sciences, 24 (2023) 18, Seite 13981
  • Sprache: Englisch
  • DOI: 10.3390/ijms241813981
  • ISSN: 1422-0067
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Lysine-specific demethylase 1 (LSD1) is highly expressed in many cancer types and strongly associated with cancer progression and metastasis. Circular RNAs (circRNAs) are produced by back-splicing and influence the interactive RNA network by microRNA and protein sponging. In the present study, we aimedto identify circRNAs that derive from the LSD1-encoding KDM1A gene, and to investigate their potential to be released and uptaken by lung cancer versus non-cancer epithelial cells. We identified four circLSD1-RNAs by RT-PCR with divergent primers, followed by sequencing. The expression level of circLSD1-RNAs was then studied by quantitative PCR on cellular and extracellular fractions of lung cancer (PC9) and non-cancer primary small airway epithelial (PSAE) cells. Moreover, we established the transgenic overexpression of circLSD1-RNAs. We show that circLSD1-RNAs are primarily located in the cytoplasm, but are packaged and released from lung cancer and non-cancer cells by extracellular vesicles (EVs) and ribonucleoprotein (RNP) complexes, respectively. Proteomics demonstrated a different protein pattern of EV fractions released from PC9 versus PSAE cells. Importantly, released circLSD1-RNAs were differently taken up by PSAE and PC9 cells. In conclusion, our findings provide primary evidence that circLSD1-RNAs participate in the intercellular communication of lung cancer cells with the tumor environment.</jats:p>
  • Zugangsstatus: Freier Zugang