Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Molecular Testing in CML between Old and New Methods: Are We at a Turning Point?
Beteiligte:
Soverini, Simona;
Bernardi, Simona;
Galimberti, Sara
Erschienen:
MDPI AG, 2020
Erschienen in:
Journal of Clinical Medicine, 9 (2020) 12, Seite 3865
Sprache:
Englisch
DOI:
10.3390/jcm9123865
ISSN:
2077-0383
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>Molecular monitoring of minimal residual disease (MRD) and BCR-ABL1 kinase domain (KD) mutation testing have a well consolidated role in the routine management of chronic myeloid leukemia (CML) patients, as they provide precious information for therapeutic decision-making. Molecular response levels are used to define whether a patient has an “optimal”, “warning”, or “failure” response to tyrosine kinase inhibitor (TKI) therapy. Mutation status may be useful to decide whether TKI therapy should be changed and which alternative TKI (or TKIs) are most likely to be effective. Real-time quantitative polymerase chain reaction (RQ-qPCR) and Sanger sequencing are currently the gold standard for molecular response monitoring and mutation testing, respectively. However, in recent years, novel technologies such as digital PCR (dPCR) and next-generation sequencing (NGS) have been evaluated. Here, we critically describe the main features of these old and novel technologies, provide an overview of the recently published studies assessing the potential clinical value of dPCR and NGS, and discuss how the state of the art might evolve in the next years.</jats:p>