Impact of ITS-Based Sequencing on Antifungal Treatment of Patients with Suspected Invasive Fungal Infections

Medientyp: E-Artikel

Titel: Impact of ITS-Based Sequencing on Antifungal Treatment of Patients with Suspected Invasive Fungal Infections

Beteiligte: Guenter, Sara; Gorkiewicz, Gregor; Halwachs, Bettina; Kashofer, Karl; Thueringer, Andrea; Wurm, Phillip; Zollner-Schwetz, Ines; Valentin, Thomas; Prattes, Juergen; Wunsch, Stefanie; Ullrich, Elisabeth; Zurl, Christoph; Hoenigl, Martin; Krause, Robert

Erschienen: MDPI AG, 2020

Sprache: Englisch

DOI: 10.3390/jof6020043

ISSN: 2309-608X

Schlagwörter: Plant Science ; Ecology, Evolution, Behavior and Systematics ; Microbiology (medical)

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Beschreibung: Molecular techniques including the sequencing of fungal-specific DNA targets are increasingly used in the diagnosis of suspected invasive fungal infections. In contrast to established biomarkers like galactomannan or 1-3-β-d-glucan, the clinical impact of these methods remains unknown. We retrospectively investigated the impact of ITS1-sequencing on antifungal treatment strategies in 71 patients (81 samples) with suspected invasive fungal infections. ITS-sequencing either confirmed already ongoing antifungal therapy (19/71 patients, 27%), led to a change in antifungal therapy (11/71, 15%) or supported the decision to withhold antifungal treatment (34/71, 48%) (in seven of 71 patients, ITS-sequencing results were obtained postmortem). ITS-sequencing results led to a change in antifungal therapy in a relevant proportion of patients, while it confirmed therapeutic strategies in the majority. Therefore, ITS-sequencing was a useful adjunct to other fungal diagnostic measures in our cohort.

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