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Medientyp:
E-Artikel
Titel:
Optimization of Lipopeptide Biosurfactant Production by Salibacterium sp. 4CTb in Batch Stirred-Tank Bioreactors
Beteiligte:
Márquez-Villa, José Martín;
Mateos-Díaz, Juan Carlos;
Rodríguez-González, Jorge Alberto;
Camacho-Ruíz, Rosa María
Erschienen:
MDPI AG, 2022
Erschienen in:
Microorganisms, 10 (2022) 5, Seite 983
Sprache:
Englisch
DOI:
10.3390/microorganisms10050983
ISSN:
2076-2607
Entstehung:
Anmerkungen:
Beschreibung:
Halophilic microorganisms are potentially capable as platforms to produce low-cost biosurfactants. However, the robustness of bioprocesses is still a challenge and, therefore, it is essential to understand the effects of microbiological culture conditions through bioreactor engineering. Based on a design of experiments (DOE) and a response surface methodology (RSM) tailored and taken from the literature, the present work focuses on the evaluation of a composite central design (CCD) under batch cultures in stirred-tank bioreactors with the halophilic bacteria Salibacterium sp. 4CTb in order to determine the operative conditions that favor mass transfer and optimize the production of a lipopeptide. The results obtained showed profiles highlighting the most favorable culture conditions, which lead to an emulsification index (E24%) higher than 70%. Moreover, through the behavior of dissolved oxygen (DO), it was possible to experimentally evaluate the higher volumetric coefficient of mass transfer in the presence of lipopeptide (kLa = 31 1/h) as a key criterion for the synthesis of the biosurfactant on further cell expansion.