> Detailanzeige

Idzko, Marco; Panther, Elisabeth; Stratz, Christian; Müller, Tobias; Bayer, Hannes; Zissel, Gernot; Dürk, Thorsten; Sorichter, Stephan; Di Virgilio, Francesco; Geissler, Michael; Fiebich, Bernd; Herouy, Yared; Elsner, Peter; Norgauer, Johannes; Ferrari, Davide

The Serotoninergic Receptors of Human Dendritic Cells: Identification and Coupling to Cytokine Release

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: The Serotoninergic Receptors of Human Dendritic Cells: Identification and Coupling to Cytokine Release
  • Beteiligte: Idzko, Marco; Panther, Elisabeth; Stratz, Christian; Müller, Tobias; Bayer, Hannes; Zissel, Gernot; Dürk, Thorsten; Sorichter, Stephan; Di Virgilio, Francesco; Geissler, Michael; Fiebich, Bernd; Herouy, Yared; Elsner, Peter; Norgauer, Johannes; Ferrari, Davide
  • Erschienen: The American Association of Immunologists, 2004
  • Erschienen in: The Journal of Immunology
  • Sprache: Englisch
  • DOI: 10.4049/jimmunol.172.10.6011
  • ISSN: 0022-1767; 1550-6606
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title> <jats:p>The neurotransmitter 5-hydroxytryptamine (5-HT), commonly known as serotonin, is stored at peripheral sites in mast cells and released from this peripheral source upon IgE cross-linking. In this study, we investigated the expression of serotoninergic receptors (5-HTR), the signaling pathway, and biological activity of 5-HT on human dendritic cells (DC), showing that immature and mature DC expressed mRNA for different serotoninergic receptors. Thereby, the mRNA of 5-HTR1B, 5-HTR1E, 5-HTR2A, 5-HTR2B, one splicing variant of the 5-HTR3, 5-HTR4, and 5-HTR7 receptors were detected. Immature DC preferentially expressed mRNA for the heptahelical 5-HTR1B, 5-HTR1E, and 5-HTR2B receptors, while mature DC mostly expressed 5-HTR4 and 5-HTR7. The mRNA expression level of the ligand-gated cation channel 5-HTR3 and the heptahelical 5-HTR2A did not significantly change during maturation. Isotype-selective receptor agonists allowed us to show that 5-HT stimulated 5-HTR3-dependent Ca2+ influx in immature and mature DC. Moreover, we revealed that 5-HTR1 and 5-HTR2 receptor stimulation induced intracellular Ca2+ mobilization via Gi/o proteins in immature, but not mature, DC. Activation of 5-HTR4 and 5-HTR7 induced cAMP elevation in mature DC. Functional studies indicated that activation of 5-HTR4 and 5-HTR7 enhanced the release of the cytokines IL-1β and IL-8, while reducing the secretion of IL-12 and TNF-α in mature DC. In summary, our study shows that 5-HT stimulated, in a maturation-dependent manner, different signaling pathways in DC. These data point to a role for 5-HT in regulating the immune response at peripheral sites.</jats:p>
  • Zugangsstatus: Freier Zugang