Beschreibung:
<jats:p>Amperometric biosensors were fabricated by immobilizing alcohol oxidases (AOX) from two different sources onto glutaraldehyde (GA)-activated supports. Alcohol oxidases from<jats:italic>Hansenula</jats:italic>sp. and from<jats:italic>Pichia pastoris</jats:italic>were employed for immobilization. The biosensor with AOX from<jats:italic>Hansenula</jats:italic>sp. showed a linear response to ethanol in the concentration range of 0.1-0.6 mM with a sensitivity of 88.534 µA mM<jats:sup>-1</jats:sup>cm<jats:sup>-2</jats:sup>and a detection limit of 0.1 mM (S/N=3). In comparison, the biosensor with AOX from<jats:italic>P. pastoris</jats:italic>showed a linear response from 0.1-0.5 mM ethanol with a sensitivity of 76.886 µA mM<jats:sup>-1</jats:sup>cm<jats:sup>-2</jats:sup>and a detection limit of 0.1 mM. The study of stability of biosensors revealed that after 90 measurements, the biosensor with AOX from<jats:italic>Hansenula</jats:italic>sp. retained 97% of its original current response whereas the current response of the biosensor with AOX from<jats:italic>P. pastoris</jats:italic>decreased to 81% of its initial value. The biosensor with AOX from<jats:italic>Hansenula</jats:italic>sp. demonstrated slightly higher sensitivity and stability than the biosensor with AOX from<jats:italic>P. pastoris</jats:italic>.</jats:p>