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Quaresma, Carla Holandino; Melo, Michelle Nonato de Oliveira; Batista, João Vitor da Costa; Da Veiga, Venicio Féo; Oliveira, Adriana Passos; De Oliveira, Cassiana Santos; Grazi, Mirio; Ramm, Hartmut; Torre, Claudia Dalla; Schaller, Gerhard; Scherr, Claudia; Kokornaczyk, Maria Olga; Urech, Konrad; Weissenstein, Ulrike; Baumgartner, Stephan

Viscum album homeopathic tinctures: phytochemical profile and antiproliferative activity

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  • Medientyp: E-Artikel
  • Titel: Viscum album homeopathic tinctures: phytochemical profile and antiproliferative activity
  • Beteiligte: Quaresma, Carla Holandino; Melo, Michelle Nonato de Oliveira; Batista, João Vitor da Costa; Da Veiga, Venicio Féo; Oliveira, Adriana Passos; De Oliveira, Cassiana Santos; Grazi, Mirio; Ramm, Hartmut; Torre, Claudia Dalla; Schaller, Gerhard; Scherr, Claudia; Kokornaczyk, Maria Olga; Urech, Konrad; Weissenstein, Ulrike; Baumgartner, Stephan
  • Erschienen: International Journal of High Dilution Research - IJHDR, 2021
  • Erschienen in: International Journal of High Dilution Research - ISSN 1982-6206
  • Sprache: Nicht zu entscheiden
  • DOI: 10.51910/ijhdr.v17i2.918
  • ISSN: 1982-6206
  • Schlagwörter: Complementary and alternative medicine
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Background: The mistletoe Viscum album L. (Santalaceae) is a semi-parasitic plant that grows on different host trees. Although V. album antitumor activity is mainly associated with aqueous preparations, recently we showed antitumoral effects of homeopathic V. album tinctures containing also the ethanolic soluble compounds [1]. &#x0D; Aims: to analyze the phytochemical profile of different Viscum album homeopathic tinctures as well as their in vitro effects in tumoral cells. &#x0D; Methodology: The Viscum album samples (leaves, stems and berries) were collected in 2016 and 2017 (summer), and in 2018 (winter) at different locations in Switzerland. After harvest, they were immediately submitted to ethanolic extraction (45% V/V) using homeopathic methodology [2, 3]. The following mother tinctures (MT) were prepared: V. album ssp. album growing on Malus domestica, Quercus sp. and Ulmus sp., V. album ssp. austriacum from Pinus sylvestris, and V. album ssp. abietis from Abies alba. The homeopathic dynamizations (1-3dH; 6, 12, 30dH) were prepared with the respective MT. The phytochemical profiles were analysed by High Performance Liquid Chromatography tandem Mass Spectroscopy, HPLC and Thin Layer Chromatography. MT prepared in 2016 were also submitted to the Pfeiffer’s circular chromatography (PCC) [4]. The proliferation assay was performed by WST-1 [5] after incubation of tumoral cells (Yoshida and Molt-4) with non-dynamized (0.5 to 0.05% v/v) and dynamized MT. Apoptosis/necrosis was measured by flow cytometer (FACS) using Annexin V-FITC [5], and the cellular morphological aspects were analysed by light microscope. &#x0D; Results and Discussion: The chemical analyses of MT identified the presence of phenolic acids, flavonoids, lignans, as principal compounds. Besides, the HPLC indicated higher viscotoxin concentration in the summer harvest of Abies alba, Malus domestica and Quercus (897, 475, 219 ?g/mL), respectively. The PCC methodology permitted the MT differentiation. The higher levels of cellular mortality were attribute to Abies alba, Malus domestica and Quercus (p</jats:p>