Insights into the Molecular Architecture of the 26S Proteasome

Medientyp: E-Artikel
Titel: Insights into the Molecular Architecture of the 26S Proteasome
Beteiligte: Nickell, Stephan; Beck, Florian; Scheres, Sjors H. W.; Korinek, Andreas; Förster, Friedrich; Lasker, Keren; Mihalache, Oana; Sun, Na; Nagy, István; Sali, Andrej; Plitzko, Jürgen M.; Carazo, Jose-Maria; Mann, Matthias; Baumeister, Wolfgang; Varshavsky, Alexander
Erschienen: National Academy of Sciences, 2009
Erschienen in: Proceedings of the National Academy of Sciences of the United States of America
Sprache: Englisch
ISSN: 0027-8424
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Beschreibung: <p>Cryo-electron microscopy in conjunction with advanced image analysis was used to analyze the structure of the 26S proteasome and to elucidate its variable features. We have been able to outline the boundaries of the ATPase module in the "base" part of the regulatory complex that can vary in its position and orientation relative to the 20S core particle. This variation is consistent with the "wobbling" model that was previously proposed to explain the role of the regulatory complex in opening the gate in the a-rings of the core particle. In addition, a variable mass near the mouth of the ATPase ring has been identified as Rpn10, a multiubiquitin receptor, by correlating the electron microscopy data with quantitative mass spectrometry.</p>
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