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Medientyp:
E-Artikel
Titel:
Comparison of Activities of the Host-Specific Toxin of Helminthosporium maydis, Race T, and a Synthetic $\text{C}_{41}$ Analog
Beteiligte:
Daly, J. M.;
Kono, Y.;
Knoche, Herman W.;
Takeuchi, S.
Erschienen:
American Society of Plant Physiologists, 1983
Erschienen in:
Plant Physiology, 73 (1983) 2, Seite 440-444
Sprache:
Englisch
ISSN:
0032-0889;
1532-2548
Entstehung:
Anmerkungen:
Beschreibung:
It previously had been proposed that the host-selective toxin of Helminthosporium maydis race T consists of a series of unusual linear ($\text{C}_{35}$ to $\text{C}_{45}$)polyketols, of equal toxicity on a weight or molar ($10^{-8}-10^{-9}$) basis. Previous laboratory synthesis of T-toxin analogs was limited to shorter (C15 to $\text{C}_{26}$) versions which possessed the requisite specificity for susceptible corn (Zea mays) but were less toxic on a weight or molar ($10^{-6}-10^{-7}$) basis. In the present study, a $\text{C}_{41}$ analog with four β-ketol units spaced by CH2 bridges as in native toxin has been synthesized. On a weight or molar basis, it is as effective as native toxin or its purified components in stimulating NADH oxidation of mitochondria from susceptible corn, thus providing firm evidence for the correctness of the proposed structures of T-toxin. Additional support derives from the observation that C24 and $\text{C}_{26}$ analogs with -$(\text{CH}_{2})_{4}$- and -$(\text{CH}_{2})_{6}$- bridges between ketol groups are not as effective in stimulating NADH oxidation as are $\text{C}_{23}$ and C25 analogs with the -$(\text{CH}_{2})_{3}$- and -$(\text{CH}_{2})_{5}$- bridges of native T-toxin. It was calculated that a single molecule of the $\text{C}_{41}$ analog is at least 300 times more effective in stimulating mitochondrial oxidation than a molecule of the $\text{C}_{23}$ or C25 analogs. This emphasizes the importance of chain length for toxicity, perhaps through perturbation of membrane functions of mitochondria and/or chloroplasts.