Chikweto, Alfred;
Sharma, Ravindra N.;
Tiwari, Keshaw P.;
Verma, Shiv K.;
Calero-Bernal, Rafael;
Jiang, Tiantian;
Su, Chunlei;
Kwok, Oliver C.;
Dubey, Jitender P.
ISOLATION AND RFLP GENOTYPING OF TOXOPLASMA GONDII IN FREE-RANGE CHICKENS (GALLUS DOMESTICUS) IN GRENADA, WEST INDIES, REVEALED WIDESPREAD AND DOMINANCE OF CLONAL TYPE III PARASITES
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
ISOLATION AND RFLP GENOTYPING OF TOXOPLASMA GONDII IN FREE-RANGE CHICKENS (GALLUS DOMESTICUS) IN GRENADA, WEST INDIES, REVEALED WIDESPREAD AND DOMINANCE OF CLONAL TYPE III PARASITES
Beteiligte:
Chikweto, Alfred;
Sharma, Ravindra N.;
Tiwari, Keshaw P.;
Verma, Shiv K.;
Calero-Bernal, Rafael;
Jiang, Tiantian;
Su, Chunlei;
Kwok, Oliver C.;
Dubey, Jitender P.
Erschienen:
American Society of Parasitologists, 2017
Erschienen in:
The Journal of Parasitology, 103 (2017) 1, Seite 52-55
Beschreibung:
The objectives of the present cross-sectional study were to isolate and genotype Toxoplasma gondii in free-range chickens from Grenada, West Indies. Using the modified agglutination test, antibodies to T. gondii were found in 39 (26.9%) of 145 free-range chickens with titers of 25 in 7 chickens, 50 in 6 chickens, 100 in 2 chickens, and 200 or higher in 24 chickens. The hearts of the 39 seropositive chickens were bioassayed in mice; viable T. gondii was isolated from 20 and further propagated in cell culture. Genotyping of T. gondii DNA extracted from cell-cultured tachyzoites using the 10 PCR-restriction fragment length polymorphism (RFLP) markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed 4 genotypes, including ToxoDB PCR-RFLP no. 2 (Type III), no. 7, no. 13, and no. 259 (new). These results indicated that T. gondii population genetics in free-range chickens seems to be moderately diverse with ToxoDB no. 2 (Type III) as the most frequent (15/20 = 75%) compared to other genotypes in Grenada.