Ivanchenko, Maria G.;
Lerner, Jennifer P.;
McCormick, Renee S.;
Toumadje, Arazdordi;
Allen, Brady;
Fischer, Kay;
Hedstrom, Olaf;
Helmrich, Angela;
Barnes, David W.;
Bayne, Christopher J.
Continuous in vitro Propagation and Differentiation of Cultures of the Intramolluscan Stages of the Human Parasite Schistosoma mansoni
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Medientyp:
E-Artikel
Titel:
Continuous in vitro Propagation and Differentiation of Cultures of the Intramolluscan Stages of the Human Parasite Schistosoma mansoni
Beteiligte:
Ivanchenko, Maria G.;
Lerner, Jennifer P.;
McCormick, Renee S.;
Toumadje, Arazdordi;
Allen, Brady;
Fischer, Kay;
Hedstrom, Olaf;
Helmrich, Angela;
Barnes, David W.;
Bayne, Christopher J.
Erschienen:
National Academy of Sciences of the United States of America, 1999
Erschienen in:Proceedings of the National Academy of Sciences of the United States of America
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
<p>The metazoan parasitic blood flukes, Schistosoma spp., infect over 200 million people worldwide and cause extensive human morbidity and mortality. Research strategies for development of anti-schistosomal agents are impeded by the organism's complex molluscan-mammalian life cycle, which limits experimental approaches and availability of materials. We derived long-term continuously proliferative cultures of Schistosoma mansoni sporocysts capable of generating cercariae in vitro. Cultured organisms retained the ability to parasitize the host, and they exhibited developmental regulation of candidate stage-specific genes in the host-free culture system. Evidence for expression of a reverse transcriptase also was found in the cultured organisms, pointing to this activity as a possible mechanistic contributor to the dynamic relationship between the parasite and its hosts. Continuous in vitro propagation of the asexual sporocyst stage allows isolation of clonally derived parasite populations and provides a means to study schistosomal molecular genetics, metabolism, and evasion of host defenses.</p>