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Medientyp:
E-Artikel
Titel:
Cytoplasmic RNA Modulators of an Inside-Out Signal-Transduction Cascade
Beteiligte:
Blind, Michael;
Kolanus, Waldemar;
Famulok, Michael
Erschienen:
National Academy of Sciences of the United States of America, 1999
Erschienen in:
Proceedings of the National Academy of Sciences of the United States of America, 96 (1999) 7, Seite 3606-3610
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
A vaccinia virus-based RNA expression system enabled high-level cytoplasmic expression of RNA aptamers directed against the intracellular domain of the β 2 integrin LFA-1, a transmembrane protein that mediates cell adhesion to intercellular adhesion molecule-1 (ICAM-1). In two different cell types, cytoplasmic expression of integrin-binding aptamers reduced inducible cell adhesion to ICAM-1. The aptamers specifically target, and thereby define, a functional cytoplasmic subdomain important for the regulation of cell adhesion in leukocytes. Our approach of aptamer-controlled blocking of signaling pathways in vivo could potentially be applied wherever targeted modulation of a signal-transduction cascade is desired.