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Medientyp:
E-Artikel
Titel:
DNA Polymerase Activities in Differentiating Mouse Neuroblastoma N-18 Cells
Beteiligte:
Bhattacharya, Prabir;
Basu, Subhash
Erschienen:
National Academy of Sciences of the United States of America, 1978
Erschienen in:
Proceedings of the National Academy of Sciences of the United States of America, 75 (1978) 3, Seite 1289-1293
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
The activities of two DNA polymerases (DNA nucleotidyltransferases) were characterized in mouse neuroblastoma clone N-18 on the basis of their apparent molecular weights (determined by sucrose density gradient centrifugation: polymerase-α , 7.5-8 S; polymerase-β , 3-4 S) and relative inhibition by sulfydryl-blocking agents. N-Ethylmaleimide (10 mM) and iodoacetamide (1.5 mM) inhibited DNA polymerase-α activity completely, whereas only 35-40% inhibition was observed for DNA polymerase-β under similar conditions. DNA polymerase-α activity was reduced 50-70% in N-18 cells that had been induced to differentiate by 4 μ M bromodeoxyuridine, and the low molecular weight DNA polymerase-β activity remain unchanged. With activated calf thymus DNA as template, only DNA polymerase-α activity was stimulated in the presence of added ribonucleotides and purified Escherichia coli RNA polymerase.