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Medientyp:
E-Artikel
Titel:
Biological Significance of Carbohydrate Chains on Monoclonal Antibodies
Beteiligte:
Nose, Masato;
Wigzell, Hans
Erschienen:
National Academy of Sciences of the United States of America, 1983
Erschienen in:Proceedings of the National Academy of Sciences of the United States of America
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
<p>We have prepared monoclonal hapten-specific mouse IgG2b antibodies depleted of asparagine-linked carbohydrate chains by treating the hybridoma cells with tunicamycin. The carbohydrate-deficient antibodies behaved in an identical manner to the normal antibodies with regard to fine antigen-binding reactivity (a Fab fragment feature) and protein A binding capacity [a feature requiring integrity at the C<sub>H</sub>2 and C<sub>H</sub>3 domain-interaction regions in the constant region of the heavy chain (C<sub>H</sub>)]. However, they lost the ability to activate complement, to bind to Fc receptors on macrophages, and to induce antibody-dependent cellular cytotoxicity. Furthermore, antigen--antibody complexes produced from such carbohydrate-deficient antibodies failed to be eliminated rapidly from the circulation. We conclude that removal of carbohydrate chains from IgG molecules may have a profound and highly select impact on the biological activity to these antibodies.</p>