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Medientyp:
E-Artikel
Titel:
Molecular analysis of tri-mutant alleles in tomato indicates the Tri locus is the gene encoding the apoprotein of phytochrome B1
Beteiligte:
Kerckhoffs, L.H.J.;
Van Tuinen, A.;
Hauser, B.A.;
Cordonnier-Pratt, M.-M.;
Nagatani, A.;
Koornneef, M.;
Pratt, L.H.;
Kendrick, R.E.
Erschienen:
Springer-Verlag, 1996
Erschienen in:
Planta, 199 (1996) 1, Seite 152-157
Sprache:
Englisch
ISSN:
1432-2048;
0032-0935
Entstehung:
Anmerkungen:
Beschreibung:
Four monogenic recessive tomato (Lycopersicon esculentum Mill.) mutants at the temporarily red light-insensitive (tri) locus (tri1, tri2 in the genetic background breeding line GT; tri3, tri4 in the genetic background cultivar Moneymaker) were studied. These mutants had slightly longer hypocotyls under white light than the wild type (WT). Western-blot analysis showed that the tri1 mutant was deficient in a relatively light-stable phytochrome apoprotein (116 kDa) that was recognized in the WT by an antibody to tobacco phytochrome B; tri2 had a 166-kDa band reduced in abundance; and tri2 and tri4 had bands reduced in molecular mass, approx. 105 and 95 kDa, respectively. These patterns were also found in light-grown plants. Northern-blot analysis for PHYB1 mRNA showed for tri2 a transcript approx. 2 kb larger, for tri4, a transcript of WT size, but much reduced in abundance and for tri1 and tri3 transcripts equivalent in size and abundance to WT. In these mutants the transcripts of other members of the tomato phytochrome gene family (PHYA, PHYB2, PHYE, PHYF) were indistinguishable in size and abundance from WT. Thus, it appears that the tri locus specifically affects PHYB1 gene expression. Unlike phytochrome-B mutants in other plants, de-etiolated seedlings of the tri mutans exhibited normal responses to end-of-day far-red (EODFR) light and supplementary far-red light during the day. Since the holophytochromes of types B1 and B2 (phyB1 and phyB2) are closely related, it is proposed that there might be redundancy between them for these responses.