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Medientyp:
E-Artikel
Titel:
The N-End Rule is Mediated by the UBC2(RAD6) Ubiquitin-Conjugating Enzyme
Beteiligte:
Dohmen, R. Jurgen;
Madura, Kiran;
Bartel, Bonnie;
Varshavsky, Alexander
Erschienen:
National Academy of Sciences of the United States of America, 1991
Erschienen in:Proceedings of the National Academy of Sciences of the United States of America
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
<p>The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all organisms examined, from mammals to bacteria. We show that UBC2(RAD6), one of at least seven ubiquitin-conjugating enzymes in the yeast Saccharomyces cerevisiae, is essential for multiubiquitination and degradation of the N-end rule substrates. We also show that UBC2 is physically associated with UBR1, the recognition component of the N-end rule pathway. These results indicate that some of the UBC2 functions, which include DNA repair, induced mutagenesis, sporulation, and regulation of retrotransposition, are mediated by protein degradation via the N-end rule pathway.</p>