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Medientyp:
E-Artikel
Titel:
Two Human Glutamate Decarboxylases, 65-kDa GAD and 67-kDa GAD, are Each Encoded by a Single Gene
Beteiligte:
Bu, Ding-Fang;
Erlander, Mark G.;
Hitz, Benjamin C.;
Kaufman, Daniel L.;
Wagner-McPherson, Caryn B.;
Evans, Glen A.;
Tobin, Allan J.
Erschienen:
National Academy of Sciences of the United States of America, 1992
Erschienen in:Proceedings of the National Academy of Sciences of the United States of America
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
<p>We report the isolation and sequencing of cDNAs encoding two human glutamate decarboxylases (GADs; L-glutamate 1-carboxy-lyase, EC 4.1.1.15), GAD<sub>65</sub>and GAD<sub>67</sub>. Human GAD<sub>65</sub>cDNA encodes a M<sub>r</sub>65,000 polypeptide, with 585 amino acid residues, whereas human GAD<sub>67</sub>encodes a M<sub>r</sub>67,000 polypeptide, with 594 amino acid residues. Both cDNAs direct the synthesis of enzymatically active GADs in bacterial expression systems. Each cDNA hybridizes to a single species of brain mRNA and to a specific set of restriction fragments in human genomic DNA. In situ hybridization of fluorescently labeled GAD probes to human chromosomes localizes the human GAD<sub>65</sub>gene to chromosome 10p11.23 and the human GAD<sub>67</sub>gene to chromosome 2q31. We conclude that GAD<sub>65</sub>and GAD<sub>67</sub>each derive from a single separate gene. The cDNAs we describe should allow the bacterial production of test antigens for the diagnosis and prediction of insulin-dependent diabetes mellitus.</p>