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Medientyp:
E-Artikel
Titel:
Cloning of the Low-Affinity Murine Granulocyte-Macrophage Colony- Stimulating Factor Receptor and Reconstitution of a High-Affinity Receptor Complex
Beteiligte:
Park, Linda S.;
Martin, Unja;
Sorensen, Rick;
Luhr, Sean;
Morrissey, Philip J.;
Cosman, David;
Larsen, Alf
Erschienen:
National Academy of Sciences of the United States of America, 1992
Erschienen in:
Proceedings of the National Academy of Sciences of the United States of America, 89 (1992) 10, Seite 4295-4299
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
A cDNA clone (clone 71) that encodes a low-affinity receptor for murine granulocyte-macrophage colony-stimulating factor (GM-CSF) has been isolated by direct expression. This molecule is the homologue of the human GM-CSF receptor α subunit, although homology between these molecules is surprisingly low (less than 35% amino acid identity). The cDNA encodes a polypeptide of 387 amino acids, which contains the conserved features of the hematopoietin receptor superfamily. When expressed in COS-7 cells, this clone encodes a protein that binds radiolabeled murine GM-CSF with low affinity. Coexpression of clone 71 with a cDNA corresponding to a low-affinity interleukin 3 (IL-3) receptor (AIC2A) did not alter the affinity of binding of either GM-CSF or IL-3. However, coexpression of clone 71 with the IL-3 receptor-related cDNA AIC2B generated high-affinity binding sites for murine GM-CSF but not murine IL-3. These studies show that clone 71 and AIC2B are capable of forming an αβ complex capable of binding murine GM-CSF with high affinity, while AIC2A appears not to be a component of the murine GM-CSF receptor.