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Medientyp:
E-Artikel
Titel:
An Investigation of Factors which Influence the Production of Abnormal Terpenoids by Callus Cultures of Andrographis paniculata Nees
Beteiligte:
BUTCHER, D. N.;
CONNOLLY, J. D.
Erschienen:
OXFORD AT THE CLARENDON PRESS, 1971
Erschienen in:Journal of Experimental Botany
Sprache:
Englisch
ISSN:
0022-0957;
1460-2431
Entstehung:
Anmerkungen:
Beschreibung:
<p>Callus and suspension cultures of Andrographis paniculata have been shown to produce three new sesquiterpene lactones which have been named paniculides A, B, and C and not the andrographolides (diterpenes) produced by the intact plant. Cultures derived from leaves, stems, hypocotyls, roots, and embryos accumulated paniculides, but no andrographolides were detected. Conversely andrographolides, but no paniculides were detected in extracts from leaves, stems and roots of intact seedlings. Modifications of the medium including the use of different synthetic auxins and the replacement of coconut milk with kinetin and casein hydrolysate did not influence paniculide production by the hypocotyl cultures. In addition an established root isolate cultured for 9 months on a simple synthetic medium without auxin, meso-inositol or coconut milk still synthesized significant quantities of paniculides. Diurnal fluctuations of temperature and light did not influence the pattern of compounds produced although cultures grown in complete darkness produced the individual paniculides in different proportions. Possible explanations for these findings are discussed.</p>