Lotz, Margaret M.;
Andrews,, Charles W.;
Korzelius, Cynthia A.;
Lee, Edward C.;
Steele,, Glenn D.;
Clarke, Astrid;
Mercurio, Arthur M.
Decreased Expression of Mac-2 (Carbohydrate Binding Protein 35) and Loss of Its Nuclear Localization are Associated with the Neoplastic Progression of Colon Carcinoma
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Medientyp:
E-Artikel
Titel:
Decreased Expression of Mac-2 (Carbohydrate Binding Protein 35) and Loss of Its Nuclear Localization are Associated with the Neoplastic Progression of Colon Carcinoma
Beteiligte:
Lotz, Margaret M.;
Andrews,, Charles W.;
Korzelius, Cynthia A.;
Lee, Edward C.;
Steele,, Glenn D.;
Clarke, Astrid;
Mercurio, Arthur M.
Erschienen:
National Academy of Sciences of the United States of America, 1993
Erschienen in:Proceedings of the National Academy of Sciences of the United States of America
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
<p>The Mac-2 lectin (carbohydrate binding protein 35) is a soluble, 32- to 35-kDa phosphoprotein that binds galactose-containing glycoconjugates. We report here that the colonic epithelium is a major site of Mac-2 expression in vivo based on immunohistochemistry of human tissue specimens. In this epithelium, proliferating cells at the base of the crypts do not express Mac-2 but its expression increases with differentiation along the crypt-to-surface axis. Mac-2 expression is concentrated in the nuclei of these differentiated epithelial cells. The progression from normal mucosa to adenoma to carcinoma is associated with significant changes in Mac-2 nuclear localization and expression. In all adenomas (9/9) and carcinomas (13/13) examined, Mac-2 was not present in the nucleus but was localized in the cytoplasm. Sequencing of Mac-2 cDNAs from normal mucosa and carcinoma revealed no specific mutations that could account for this loss of nuclear localization. We also observed a 5- to 10-fold decrease in Mac-2 mRNA levels in cancer compared to normal mucosa as well as a significant reduction in the amount of Mac-2 protein expressed. These observations suggest that Mac-2 exclusion from the nucleus and its decreased expression may be related to the neoplastic progression of colon cancer.</p>