Dallaire, Frédéric;
Ouellet, Nathalie;
Simard, Marie;
Bergeron, Yves;
Bergeron, Michel G.
Efficacy of Recombinant Human Granulocyte Colony-Stimulating Factor in a Murine Model of Pneumococcal Pneumonia: Effects of Lung Inflammation and Timing of Treatment
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Medientyp:
E-Artikel
Titel:
Efficacy of Recombinant Human Granulocyte Colony-Stimulating Factor in a Murine Model of Pneumococcal Pneumonia: Effects of Lung Inflammation and Timing of Treatment
Beteiligte:
Dallaire, Frédéric;
Ouellet, Nathalie;
Simard, Marie;
Bergeron, Yves;
Bergeron, Michel G.
Erschienen:
University of Chicago Press, 2001
Erschienen in:
The Journal of Infectious Diseases, 183 (2001) 1, Seite 70-77
Beschreibung:
The effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) in a murine model of pneumococcal pneumonia was examined. Intranasal inoculations were 10⁷ cfulmouse (high inoculum) and 5 x 10⁴ cfulmouse (low inoculum) of Streptococcus pneumoniae, which induced severe or mild lung inflammation, respectively. With the low inoculum, rhG-CSF significantly improved survival when initiated 24 h or 10 min before, but not when initiated 24 h after, infection. Pretreatment with rhG-CSF significantly increased myeloperoxidase (MPO) activity in lungs 8 h after the infection and increased circulating neutrophil count 24, 48, and 72 h after infection. In contrast, rhG-CSF did not improve survival of animals infected with the high inoculum and did not increase MPO activity or neutrophil count in blood over those of sham-treated controls. These data strongly suggest that the severe inflammatory response typically observed in pneumococcal pneumonia recruits a maximum number of neutrophils in the lungs and thus masks the beneficial effect of rhG-CSF.