Analysis of DNA Restriction Fragment Length Polymorphism Extends the Evidence for Breast Milk Transmission in Streptococcus agalactiae Late-Onset Neonatal Infection
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E-Artikel
Titel:
Analysis of DNA Restriction Fragment Length Polymorphism Extends the Evidence for Breast Milk Transmission in Streptococcus agalactiae Late-Onset Neonatal Infection
Beschreibung:
Analysis of restriction fragment length polymorphism (RFLP) of total DNA and of ribosomal DNA (ribotyping) was used to document four cases of Streptococcus agalactiae mother-to-infant transmission potentially associated with ingestion of infected mother's milk. Twenty strains were analyzed. Ten strains were mother-baby pairs, five from the milk of five mothers, four from their neonates with late-onset infection, and one from a colonized neonate. All mothers had early postpartum mastitis. Ten unrelated strains were studied for comparison. In each case, the two strains of each mother-baby pair produced identical RFLP patterns of total DNA. The 10 unrelated strains generated 10 different patterns, one of which, though, was observed in one of the mother-baby pairs. Ribotyping was less discriminative than total DNA RFLP analysis (6 different patterns vs. 13). These data extend the evidence for breast milk transmission in S. agalactiae lateonset neonatal infection.